CBX5 antibody

Catalog No. ABIN272193

Product Details anti-CBX5 Antibody

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of HP1-alpha/CBX5 protein. (region surrounding Lys69)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000-1/20000. Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Alternative Name: CBX5 (CBX5 Products)

Synonyms: HP1 antibody, HP1A antibody, id:ibd2026 antibody, wu:fc63e02 antibody, wu:fe47g12 antibody, wu:fi03h05 antibody, zgc:158601 antibody, CBX5 antibody, cbx5 antibody, MGC64354 antibody, 2610029O15Rik antibody, C75991 antibody, Hp1a antibody, Hp1alpha antibody, chromobox 5 antibody, chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody, chromobox 5 S homeolog antibody, CBX5 antibody, cbx5 antibody, Cbx5 antibody, cbx5.S antibody

Background: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1), HP1α, HP1β and HP1γ. As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.Synonyms: Chromobox protein homolog 5, HP1 alpha, HP1-alpha, HP1A, Heterochromatin protein 1 homolog alpha, p25

Molecular Weight: approx. 22 kDa

Gene ID: 23468

NCBI Accession: NP_001120793

UniProt: P45973