CSTF1 antibody

Catalog No. ABIN272214

Product Details anti-CSTF1 Antibody

Target: CSTF1 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSTF1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of CstF-50 protein (region surrounding Arg3)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CSTF1

Target: CSTF1 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))

Alternative Name: CSTF1 (CSTF1 Products)

Synonyms: fc22d10 antibody, zgc:66207 antibody, zgc:77560 antibody, wu:fc22d10 antibody, wu:fd16h09 antibody, MGC53063 antibody, MGC69421 antibody, MGC83790 antibody, 1700057K18Rik antibody, AI788832 antibody, CstF-50 antibody, CstFp50 antibody, cleavage stimulation factor, 3' pre-RNA, subunit 1 antibody, cleavage stimulation factor subunit 1 antibody, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa antibody, cstf1 antibody, cstf1.S antibody, CSTF1 antibody, cstf1.L antibody, Cstf1 antibody

Background: Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits of 77, 64 and 50 kDa. Heterotrimeric CstF recognizes GU and U-rich sequences located downstream of the polyadenylation site on RNA. The 50 kDa CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44 amino acid-long sequence that is repeated seven times. CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro. CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3'-end cleavage factors to form a stable complex on the pre-mRNA.Synonyms: CF-1 50 kDa subunit, CSTF 50 kDa subunit, Cleavage stimulation factor 50 kDa subunit, CstF-50, CstFp50

Molecular Weight: approx. 48 kDa

Gene ID: 1477

NCBI Accession: NP_001028693

UniProt: Q05048