MTA1 antibody

Catalog No. ABIN272222

Product Details anti-MTA1 Antibody

Target: MTA1 (Metastasis Associated 1 (MTA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of MTA1 protein. (region surrounding Lys182)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MTA1

Target: MTA1 (Metastasis Associated 1 (MTA1))

Alternative Name: MTA1 (MTA1 Products)

Synonyms: Tex22 antibody, metastasis associated 1 antibody, metastasis associated 1 L homeolog antibody, MTA1 antibody, Mta1 antibody, mta1.L antibody

Background: MTA1 (metastasis-associated protein 1) is a component of the NURD (for nucleosome remodeling and histone deacetylation) complex, which is associated with ATP-dependent chromatin-remodeling and histone deacetylase activity. MTA1 functions in conjunction with other components of NURD to mediate transcriptional repression as it facilitates the association of repressor molecules with the chromatin. Structurally, MTA1 contains a single SH3-binding motif and a zinc finger domain, along with a region similar to the corepressor protein N-Cor. MTA1 is normally expressed at low levels in various tissues and is more highly expressed in testis. Overexpression of MTA1 correlates with tumor invasion and metastasis in various carcinomas including colorectal, gastrointestinal and breast carcinomas. Elevated MTA1 levels in these tumors appears to enhance the metastases to lymph nodes, increase mammary cell motility and potentiate growth, and it may, therefore, be an indicator for assessing the potential malignancies of various tumors. A similar protein, MTA1-L1 (MTA1-like protein 1), shares more than 55 % sequence homology with MTA1 and is ubiquitously expressed.Synonyms: Metastasis-associated protein MTA1

Molecular Weight: approx. 80 kDa

Gene ID: 9112

NCBI Accession: NP_001190187

UniProt: Q13330

Pathways: Chromatin Binding