GIT2 antibody

Catalog No. ABIN272229

Product Details anti-GIT2 Antibody

Target: GIT2 (G Protein-Coupled Receptor Kinase Interactor 2 (GIT2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIT2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of GIT2 protein. (region surrounding Asp391)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GIT2

Target: GIT2 (G Protein-Coupled Receptor Kinase Interactor 2 (GIT2))

Alternative Name: GIT2 (GIT2 Products)

Synonyms: CAT-2 antibody, CAT2 antibody, cat-2 antibody, xgit2 antibody, git2 antibody, si:dkey-234h16.4 antibody, 1500036H07Rik antibody, 5830420E16Rik antibody, 6430510B20Rik antibody, 9630056M03Rik antibody, B230104M05Rik antibody, mKIAA0148 antibody, zgc:158416 antibody, GIT ArfGAP 2 antibody, G protein-coupled receptor kinase interacting ArfGAP 2 antibody, G protein-coupled receptor kinase interacting ArfGAP 2a antibody, G protein-coupled receptor kinase-interactor 2 antibody, G protein-coupled receptor kinase interacting ArfGAP 2 L homeolog antibody, G protein-coupled receptor kinase interacting ArfGAP 2b antibody, GIT2 antibody, git2 antibody, git2a antibody, Git2 antibody, git2.L antibody, git2b antibody

Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. G protein-coupled receptor kinases(GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade. GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking. GIT1 overexpression results in reduced internalization and resensitization of β2-adrenergic receptor, thus reducing β2-adrenergic receptor signaling.Synonyms: ARF GTPase-activating protein GIT2, CAT2, Cool-interacting tyrosine-phosphorylated protein 2, G protein-coupled receptor kinase-interactor 2, GRK-interacting protein 2, KIAA0148

Molecular Weight: approx. 84 kDa

Gene ID: 9815

NCBI Accession: NP_001128685

UniProt: Q14161

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling