CDKL2 antibody

Catalog No. ABIN272246

Product Details anti-CDKL2 Antibody

Target: CDKL2 (Cyclin Dependent Kinase Like 2 (CDKL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of KKIAMRE protein. (region surrounding Leu240)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CDKL2

Target: CDKL2 (Cyclin Dependent Kinase Like 2 (CDKL2))

Alternative Name: CDKL2 (CDKL2 Products)

Synonyms: KKIAMRE antibody, P56 antibody, CDKL2 antibody, 5330436L21Rik antibody, AI505225 antibody, Kkm antibody, DKFZp459L235 antibody, kkiamre antibody, cyclin dependent kinase like 2 antibody, cyclin-dependent kinase-like 2 (CDC2-related kinase) antibody, cyclin dependent kinase like 2 L homeolog antibody, CDKL2 antibody, Cdkl2 antibody, cdkl2.L antibody

Background: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated by the MAP kinases ERK 1 and ERK 2. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. The protein kinase p56 KKIAMRE is distantly related to the MAP kinase group of proteins and is closely related to p42 KKIALRE. KKIAMRE is predominantly expressed in testis, kidney, brain and lung. KKIAMRE contains the conserved MAP kinase dual phosphorylation motif in the sequence Thr-Asp-Tyr and is activated by treatment of cells by EGF. However, unlike other MAP kinases, the EGF-stimulated kinase activity does not require phosphorylation of KKIAMRE and KKIALRE in the Thr-Asp-Tyr motif.Synonyms: Cyclin-dependent kinase-like 2, KKIAMRE, Protein kinase p56 KKIAMRE, Serine/threonine-protein kinase KKIAMRE

Molecular Weight: approx. 56 kDa

Gene ID: 8999

NCBI Accession: NP_003939

UniProt: Q92772