MLX antibody
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- Target See all MLX Antibodies
- MLX (MAX-Like Protein X (MLX))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MLX antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of Mlx protein. (region surrounding Gln136)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product MLX Primary Antibody
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- Application Notes
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ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE!
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- MLX (MAX-Like Protein X (MLX))
- Alternative Name
- Max-Like Protein X (MLX Products)
- Synonyms
- MAD7 antibody, MXD7 antibody, TCFL4 antibody, bHLHd13 antibody, Tcfl4 antibody, im:7146242 antibody, wu:fb61a01 antibody, wu:fb99c01 antibody, zgc:112409 antibody, DKFZp468J1719 antibody, MLX, MAX dimerization protein antibody, MAX-like protein X antibody, max-like protein X antibody, MLX antibody, Mlx antibody, mlx antibody, LOC786043 antibody
- Background
- Max is a nuclear localized bHLH-Zip protein that forms homodimers or heterodimers with Myc family members, including Myc, Mad 1, Mad 3, Mad 4, Mxi1 and Mnt (or Rox). These dimers bind to the E-box sequence CACGTG in order to regulate cell growth, proliferation and apoptosis. Mlx (Max-like protein X) is a bHLH-Zip protein that is structurally and functionally related to Max. Like Max, Mlx is broadly expressed in many tissues and has a long half-life. Mlx also forms homodimers or heterodimers with members of the Myc family, specifically Mad 1, Mad 4 and Rox, and members of the Mondo family, to repress or activate transcription from CACGTG E-boxes. MondoA forms weak homodimers and preferentially forms heterodimers with Mlx. The MondoA/Mlx complex is primarily localized to the cytoplasm, but will translocate to the nucleus in response to leptomycin B. Mlx can also dimerize with WBSCR14, a protein involved in Williams-Beuren syndrome (WBS), to repress E-box transcription, which provides further evidence that Mlx is a critical element in a transcription factor network.Synonyms: BigMax protein, MLX, Max-like bHLHZip protein, TCFL4, Transcription factor-like protein 4
- Molecular Weight
- approx. 33 kDa
- Gene ID
- 6945
- NCBI Accession
- NP_733752
- UniProt
- Q9UH92
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