CYP2C8 antibody

Catalog No. ABIN272275

Product Details anti-CYP2C8 Antibody

Target: CYP2C8 (Cytochrome P450, Family 2, Subfamily C, Polypeptide 8 (CYP2C8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP2C8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of CYP2C8/9/18/19 protein. (region surrounding Met136)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CYP2C8

Target: CYP2C8 (Cytochrome P450, Family 2, Subfamily C, Polypeptide 8 (CYP2C8))

Alternative Name: CYP2C8 (CYP2C8 Products)

Synonyms: CPC8 antibody, CYPIIC8 antibody, MP-12/MP-20 antibody, CYP2C74 antibody, cpc8 antibody, cyp2c8 antibody, cypiic8 antibody, mp-12/mp-20 antibody, cytochrome P450 family 2 subfamily C member 8 antibody, Cytochrome P450 2C8 antibody, cytochrome P450, family 2, subfamily C, polypeptide 8 antibody, cytochrome P450 family 2 subfamily C member 8 S homeolog antibody, CYP2C8 antibody, CTN_0597 antibody, cyp2c8.1.S antibody, cyp2c8.1 antibody

Background: The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is known for its involvement in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.Cyclophilin B (CyPB) is a member of the cyclophilin family with specific N- and C-terminal extensions. Unlike CyPA, CyPB has a signal sequence leading to its translocation in the endoplasmic reticulum. CyPB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes.Synonyms: CYPIIC8, Cytochrome P450 2C8, Cytochrome P450 IIC2, Cytochrome P450 MP-12/MP-20, Cytochrome P450 form 1, S-mephenytoin 4-hydroxylase

Molecular Weight: approx. 60 kDa

Gene ID: 1558

NCBI Accession: NP_000761

UniProt: P10632