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Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody

Antigen

Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)
Synonyms KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B
Clonality Monoclonal (DCS-72)
Host
Alternatives

Mouse
Reactivity
Alternatives

Dog (Canine)
Application
Alternatives Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Catalog no. ABIN272326
Quantity 100 µg  (Lot specific)
Price 270.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics Synonyms: p27Kip1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27
Alternative name CDKN1B / KIP1
Swiss-Prot P46527
Immunogen His-tagged recombinant full length Mouse p27 protein recognizing a 27kDa protein.
Cross-Reactivity Dog (Canine), Human, Monkey, Mouse (Murine), Rat (Rattus)
Isotype IgG1  (Matching secondary antibodies)
Clone DCS-72
Description P27 associates with cyclin and cyclin-dependent kinase complexes to inhibit their kinaseactivity and contribute to the control of cell proliferation. p27 is phosphorylated on manysites, including threonine 187, in vivo, with the predominant phosphorylation site beingserine 10. The extent of serine 10 phosphorylation by proline-directed kinase is markedlyincreased in cells in the G(0)-G(1) phase of the cell cycle compared to cells in the S or Mphase. p27 concentration is regulated predominantly by post-translational mechanisms. p27 is degraded by both the ubiquitin-proteasome pathway and ubiquitinindependentproteolysis. Regulation of ubiquitin-mediated proteolysis is often achieved throughubiquitination of the targeted phosphorylated protein, which renders it more susceptibleto degradation.
Specificity The p27 kip1 functions as a Negative regulator of G1 mouse expression and has beenproposed to function as a possible mediator of TGFb induced G1 arrest. Species: Human, Mouse, Rat, Monkey and Canine (Dog). Others not tested. Add. Information: Predicted Molecular Weight: 22. 1 kDa

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Western Blot: 5-10 µg/ml. Immunoprecipitation: coprecipitates CDK4. Immunohistochemistry (Paraffin). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration Lot specific
Purification Purified
Buffer PBS containing 0.08% Sodium Azide as preservative.
Storage Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Research Area Cancer, Cell Cycle
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)", type "Antibodies"
Hosts Rabbit (47), Mouse (15), Goat (6)
Reactivities Human (61), Mouse (Murine) (21), Rat (Rattus) (19), Dog (Canine) (3), Cat (Feline) (1), Cow (Bovine) (1)
Applications Western Blotting (WB) (55), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (28), Enzyme Immunoassay (EIA) (15), Immunoprecipitation (IP) (10), ELISA (9), Immunohistochemistry (IHC) (9), Immunofluorescence (IF) (7), ELISA (Detection) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Dot Blot (Dot) (2)
Epitopes pThr187 (5), internal (2), pSer178 (2), pThr157 (2), pThr198 (2), C-Term (1), T198 (1), pS10 (1), pS178 (1), pSer10 (1), pT157 (1), pT187 (1)