Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE) (pSer22) antibody

Antigen: Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, epsilon (NFKBIE)

Synonyms: IKBE, NFKBIE

Epitope: pSer22

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN273559

Additional Information

Alternative name: IkB-epsilon (Phospho-Ser22)

Swiss-Prot: O00221

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human IkB-epsilon around the phosphorylation site of serine 22 ( I-E-S P -L-R).

Isotype: IgG  (Matching secondary antibodies)

Description: Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity: IkB-epsilon (Phospho-Ser22) antibody detects endogenous levels of IkB-epsilon only when phosphorylated at serine 22.

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100

Concentration: 100µg/100µl

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage: Store at -20℃/1 year

Restrictions: For Research Use only

Images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-ε (Phospho-Ser22) antibody (E011213).

Western blot analysis of extract from 293 cells, untreated or treated with TNF-α (20ng/ml, 15min), using IkB-ε (Ab-22) antibody (E021296, Lane 1 and 2) and IkB-ε (Phospho-Ser22) antibody (E011213, Lane 3 and 4).