Defensin beta 3 antibody (Biotin)

Catalog No. ABIN464854

Product Details anti-Defensin beta 3 Antibody (Biotin)

Target: Defensin beta 3 (DEFB3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Defensin beta 3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human BD-3

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human BD-3.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hBD-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-3. To detect hBD-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human BD-3 (LS-C104293) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-3. Western Blot: To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Defensin beta 3

Target: Defensin beta 3 (DEFB3)

Alternative Name: DEFB3 / beta Defensin 3 (DEFB3 Products)

Synonyms: BD-3 antibody, DEFB-3 antibody, DEFB103 antibody, DEFB3 antibody, HBD3 antibody, HBP-3 antibody, HBP3 antibody, hBD-3 antibody, BNBD-3 antibody, BNBD-6 antibody, BNDB-3 antibody, BNDB-6 antibody, BNDB3 antibody, DEFB103A antibody, DEFB2 antibody, DEFB6 antibody, bnbd3 antibody, defb405 antibody, defensin beta 103A antibody, defensin beta 3 antibody, beta-defensin 3 antibody, DEFB103A antibody, Defb3 antibody, DEFB3 antibody

Pathways: Production of Molecular Mediator of Immune Response