RFP antibody (Red Fluorescent Protein)

Details for Product anti-RFP Antibody No. ABIN2745401, Supplier: Log in to see
Antigen
Reactivity
Discosoma
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50
36
10
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2
1
1
Host
Mouse
95
39
14
9
1
Clonality (Clone)
Monoclonal ()
Conjugate
This RFP antibody is un-conjugated
9
7
6
3
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3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Western Blotting (WB)
133
72
61
53
33
29
7
5
4
3
2
1
1
1
1
1
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Options
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Supplier Product No.
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Purpose For biochemical analysis of RFP-tagged fusion proteins.
Clone 6G6
Isotype IgG2a
Specificity 6G6 efficiently recognizes monomeric red fluorescent proteins from Discosoma sp. derived from DsRed such as mRFP, mCherry or mPlum but no green fluorescent proteins.
Tested on mRFP, mCherry, mPlum, mOrange, mRFPruby, dsRed
Characteristics Use this mouse monoclonal antibody (MAb) against monomeric RFPs, designated as RFP antibody [6G6]. The 6G6 MAb is characterized by high affinity and specificity against the DsRed derivatives and corresponding fusion proteins. It is suitable for immunoblotting assays.
Purification Hybridoma supernatant
Alternative Name RFP (RFP Antibody Abstract)
Background Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells. In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc.) which became attractive tools for a number of applications.
Research Area Tags/Labels
Application Notes Western blot: recommended starting concentration 1/1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) antibody (ABIN2745401) Cell extract of HEK cells transiently expressing different red fluorescent proteins
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