RFP antibody (Red Fluorescent Protein)

Details for Product anti-RFP Antibody No. ABIN2745401, Supplier: Log in to see
Antigen
Reactivity
Discosoma
82
23
21
10
7
2
1
1
Host
Mouse
68
38
14
9
1
Clonality (Clone)
Monoclonal ()
Conjugate
This RFP antibody is un-conjugated
7
5
5
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
106
71
47
39
32
29
7
5
4
3
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Purpose For biochemical analysis of RFP-tagged fusion proteins.
Clone 6G6
Isotype IgG2a
Specificity 6G6 efficiently recognizes monomeric red fluorescent proteins from Discosoma sp. derived from DsRed such as mRFP, mCherry or mPlum but no green fluorescent proteins.
Tested on mRFP, mCherry, mPlum, mOrange, mRFPruby, dsRed
Characteristics Use this mouse monoclonal antibody (MAb) against monomeric RFPs, designated as RFP antibody [6G6]. The 6G6 MAb is characterized by high affinity and specificity against the DsRed derivatives and corresponding fusion proteins. It is suitable for immunoblotting assays.
Purification Hybridoma supernatant
Alternative Name RFP
Background Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells. In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc.) which became attractive tools for a number of applications.
Research Area Tags/Labels
Application Notes Western blot: recommended starting concentration 1/1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-RFP antibody (Red Fluorescent Protein) (ABIN2745401) Cell extract of HEK cells transiently expressing different red fluorescent proteins