PARP10 antibody (AA 1-907)

Catalog No. ABIN2745553

Product Details anti-PARP10 Antibody

Target: PARP10 (Poly (ADP-Ribose) Polymerase Family, Member 10 (PARP10))

Binding Specificity: AA 1-907

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This PARP10 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Recognizes human PARP-10 [ARTD10] with an epitope between aa 256-407, containing the glycine rich (G-rich) region.

Cross-Reactivity: Human

Immunogen: Recombinant human PARP-10 [ARTD10] (aa 1-907) fused to a GST-tag.

Clone: 5H11

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Cell culture supernatant containing RPMI, 10 % FCS and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: +4°C
Stable for at least 1 year after receipt when stored at +4°C.

Expiry Date: 12 months

Target Details for PARP10

Target: PARP10 (Poly (ADP-Ribose) Polymerase Family, Member 10 (PARP10))

Alternative Name: PARP-10 [ARTD10] (PARP10 Products)

Synonyms: ARTD10 antibody, PARP10 antibody, poly(ADP-ribose) polymerase family member 10 antibody, poly (ADP-ribose) polymerase family, member 10 antibody, PARP10 antibody, Parp10 antibody

Background: PARP-10 is a mono-ADP-ribosyltransferase and was initially identified as an interaction partner of the proto-oncoprotein Myc. PARP-10 possesses ubiquitin-interaction motifs, which are important to inhibit the activation of NF-kappaB and downstream target genes in response to interleukin-1beta and tumor necrosis factor-alpha. PARP-10 mono(ADP-ribosyl)ates (MARylates) NEMO (NF-kappaB essential modulator), which results in reduced NEMO polyubiquitylation and thus decreased NF-kappaB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3beta were identified and PARP-10-mediated mono(ADP-ribosyl)ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.

UniProt: Q53GL7