Histone 3 antibody (AA 1-50) (FITC)

Catalog No. ABIN2746743

Product Details anti-Histone 3 Antibody (FITC)

Target: Histone 3 (H3)

Binding Specificity: AA 1-50

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Specificity: The antibody labels a strong band of HH3 at 17 kDa in ABIN1741621 samples and in other cancer cell lines.

Cross-Reactivity: Rat (Rattus)

Characteristics: HH3- selective antibodies were generated against a peptide taken the N-terminal region of the protein. The HH3-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The anti- HH3- selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides HH3 Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for HH3 antibody is also available, please inquire before ordering.

Purification: Affinity Purified

Immunogen: Synthetic peptide taken within amino acid region 1-50 on HH3 protein.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: Major Histone H3, Histone H3-I/H3-II

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55-0.75 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Background: Histones are a family of basic proteins, which associate with nuclear DNA. Histones are essential for the folding and condensing of nuclear DNA to form nucleosomes and chromatin in eukaryotes. The compressed form of chromatin inhibits the interaction of transcription enzymes with DNA. A nucleosome consists of approximately 146 base pairs of DNA wrapped around a histone octamer to make the basic unit of chromatin structure. The Octamer is comprised of histones (H2A, H2B, H3 and H4) and about 200 bases of nucleic acid to form a nucleosome. Histones consist of a globular domain and a more flexible amino acid terminal domain. The histone tail that protrudes out from the nucleosomal surface can be modified by methylation, phosphorylation, ubiquitination and ADP ribosylation and biotinylation. These post translational modifications affect the structure of chromatin and are involved in DNA repair, mitotic and meiotic chromosome condensation and gene regulation. Histone H3 is a 17 kDa nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, and H4). Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells and has several sequence variants and post translational modification states that regulate the epigenetic control of genes. Histone H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 undergoes several different types of epigenetic modifications that influence cellular processes such as covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with Phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.

UniProt: P69150