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CD5 antibody (PE)

CD5 Reactivity: Human FACS Host: Mouse Monoclonal L17F12 PE
Catalog No. ABIN2749130
  • Target See all CD5 Antibodies
    CD5
    Reactivity
    • 221
    • 90
    • 58
    • 27
    • 22
    • 16
    • 15
    • 15
    • 13
    • 12
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    • 5
    • 2
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    Human
    Host
    • 166
    • 81
    • 56
    • 1
    Mouse
    Clonality
    • 232
    • 70
    Monoclonal
    Conjugate
    • 110
    • 38
    • 31
    • 28
    • 13
    • 8
    • 6
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    • 3
    • 3
    • 3
    • 3
    • 2
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    This CD5 antibody is conjugated to PE
    Application
    • 235
    • 109
    • 68
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    • 53
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    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody L17F12 reacts with an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
    Cross-Reactivity (Details)
    Human
    Purification
    Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Human acute lymphoblastic leukemia (ALL) T cells
    Clone
    L17F12
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product CD5 Primary Antibody
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Dunphy, Tang: "The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases." in: Archives of pathology & laboratory medicine, Vol. 131, Issue 5, pp. 748-54, (2007) (PubMed).

    Gong, Lagoo, Peters, Horvatinovich, Benz, Buckley: "Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma." in: American journal of clinical pathology, Vol. 116, Issue 6, pp. 893-7, (2001) (PubMed).

    McAlister, Davis, Pfuhl, Driscoll: "NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments." in: Protein engineering, Vol. 11, Issue 10, pp. 847-53, (1999) (PubMed).

    Shuster, Falletta, Pullen, Crist, Humphrey, Dowell, Wharam, Borowitz: "Prognostic factors in childhood T-cell acute lymphoblastic leukemia: a Pediatric Oncology Group study." in: Blood, Vol. 75, Issue 1, pp. 166-73, (1990) (PubMed).

    Engleman, Warnke, Fox, Dilley, Benike, Levy: "Studies of a human T lymphocyte antigen recognized by a monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 3, pp. 1791-5, (1981) (PubMed).

  • Target
    CD5
    Alternative Name
    CD5 (CD5 Products)
    Synonyms
    CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody
    Background
    CD5 Molecule,CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.,T1, LEU1
    Gene ID
    921
    UniProt
    P06127
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