Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

FAS antibody

FAS Reactivity: Human FACS, Func Host: Mouse Monoclonal EOS9-1 unconjugated
Catalog No. ABIN2749190
  • Target See all FAS Antibodies
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Reactivity
    • 213
    • 67
    • 62
    • 19
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 159
    • 88
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Clonality
    • 156
    • 99
    • 1
    Monoclonal
    Conjugate
    • 123
    • 27
    • 25
    • 12
    • 10
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FAS antibody is un-conjugated
    Application
    • 165
    • 116
    • 74
    • 65
    • 36
    • 35
    • 31
    • 26
    • 26
    • 20
    • 19
    • 8
    • 8
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Functional Studies (Func)
    Specificity
    The mouse monoclonal antibody EOS9.1 recognizes an extracellular epitope of CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
    Purity
    > 95 % (by SDS-PAGE)
    Endotoxin Level
    Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
    Immunogen
    P815 cells transfected with human CD95
    Clone
    EOS9-1
    Isotype
    IgM kappa
    Top Product
    Discover our top product FAS Primary Antibody
  • Application Notes
    Functional application: In vitro induction of apoptosis.
    Flow cytometry: Recommended dilution: 2-6 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4
    Preservative
    Azide free
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Matsuoka, Kim, McDonough, Bascug, Warshauer, Koreth, Cutler, Ho, Alyea, Antin, Soiffer, Ritz: "Altered regulatory T cell homeostasis in patients with CD4+ lymphopenia following allogeneic hematopoietic stem cell transplantation." in: The Journal of clinical investigation, Vol. 120, Issue 5, pp. 1479-93, (2010) (PubMed).

    Kasper, Konze, Kern, Stippel: "CD95 and TNFα-induced apoptosis in liver metastases of colorectal carcinoma." in: In vivo (Athens, Greece), Vol. 24, Issue 5, pp. 653-7, (2010) (PubMed).

    Conejo-Garcia, Benencia, Courreges, Gimotty, Khang, Buckanovich, Frauwirth, Zhang, Katsaros, Thompson, Levine, Coukos: "Ovarian carcinoma expresses the NKG2D ligand Letal and promotes the survival and expansion of CD28- antitumor T cells." in: Cancer research, Vol. 64, Issue 6, pp. 2175-82, (2004) (PubMed).

    Desbarats, Birge, Mimouni-Rongy, Weinstein, Palerme, Newell: "Fas engagement induces neurite growth through ERK activation and p35 upregulation." in: Nature cell biology, Vol. 5, Issue 2, pp. 118-25, (2003) (PubMed).

  • Target
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Alternative Name
    CD95 / Fas (FAS Products)
    Synonyms
    ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody
    Background
    Fas cell surface death receptor,CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.,FAS1, APT1, APO-1, FASTM, ALPS1A, TNFRSF6
    Gene ID
    355
    UniProt
    P25445
    Pathways
    p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Support