F-Box Protein 21 (FBXO21) (N-Term) antibody

Details for Product No. ABIN2774725, Supplier: Log in to see
Antigen
  • si:ch211-42a13.2
  • DKFZp459N226
  • FBX21
  • 2810425J22Rik
  • AU016673
  • mKIAA0875
  • F-box protein 21
  • FBXO21
  • fbxo21
  • Fbxo21
Epitope
N-Term
14
10
9
4
2
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast
66
47
29
8
8
3
2
2
2
2
1
1
1
Host
Rabbit
44
33
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
62
32
24
22
18
16
13
2
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human FBXO21
Blocking Peptide Blocking peptide for this product available: ABIN976732
Sequence KEQFRVRWPS LMKHYSPTDY VNWLEEYKVR QKAGLEARKI VASFSKRFFS
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%
Characteristics This is a rabbit polyclonal antibody against FBXO21. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name FBXO21 (FBXO21 Antibody Abstract)
Background FBXO21 contains 1 F-box domain. It is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants.
Alias Symbols: DKFZp434G058, FBX21, FLJ90233, KIAA0875, MGC26682
Protein Interaction Partner: TP53, CDKN1A, SUMO1, SMURF1, NEDD8, UBC, COPS5, COPS6, CUL1, ELAVL1, CNTNAP4, TERF2, TERF1,
Protein Size: 621
Molecular Weight 71 kDa
Gene ID 23014
NCBI Accession NM_015002, NP_055817
UniProt O94952
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Proteolysis / Ubiquitin
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 621 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-F-Box Protein 21 (FBXO21) (N-Term) antibody (ABIN2774725) WB Suggested Anti-FBXO21 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Nakayama, Kikuno, Ohara: "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs." in: Genome research, Vol. 12, Issue 11, pp. 1773-84, 2002 (PubMed).

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