HERC5 antibody (N-Term)

Catalog No. ABIN2774738

Product Details anti-HERC5 Antibody

Target: HERC5 (Hect Domain and RLD 5 (HERC5))

Binding Specificity: N-Term

Reactivity: Human, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HERC5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: CLVAELVGYR VTQIACGRWH TLAYVSDLGK VFSFGSGKDG QLGNGGTRDQ

Predicted Reactivity: Cow: 86%, Dog: 79%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against HERC5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HERC5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1024 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HERC5

Target: HERC5 (Hect Domain and RLD 5 (HERC5))

Alternative Name: HERC5 (HERC5 Products)

Synonyms: HERC5 antibody, CEB1 antibody, CEBP1 antibody, HECT and RLD domain containing E3 ubiquitin protein ligase 5 antibody, hect domain and RLD 5 antibody, HERC5 antibody

Background: HERC5 is a probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.
Alias Symbols: CEB1, CEBP1
Protein Interaction Partner: UBC, SMAD4, UBE2D1, gag, ISG15, TXNRD1, IRF3, HSPA8, CTNNB1, HERC5, SIRT7, STK38, PITX2, ERCC2, CCND1, CCNE1, CCNB1, CCNA2, NME2, CDKN1A,
Protein Size: 1024

Molecular Weight: 117 kDa

Gene ID: 51191

NCBI Accession: NM_016323, NP_057407

UniProt: Q9UII4