MARCH4 antibody (N-Term)

Catalog No. ABIN2774770

Product Details anti-MARCH4 Antibody

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GLLKCRCRML FNDLKVFLLR RPPQAPLPMH GDPQPPGLAA NNTLPALGAG

Predicted Reactivity: Dog: 92%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 85%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against MARCH4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MARCH4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 410 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MARCH4

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Alternative Name: MARCH4 (MARCH4 Products)

Synonyms: MARCH-IV antibody, RNF174 antibody, BC056494 antibody, mKIAA1399 antibody, MARCH4 antibody, si:ch211-239d11.2 antibody, membrane associated ring-CH-type finger 4 antibody, membrane-associated ring finger (C3HC4) 4 antibody, MARCH4 antibody, March4 antibody, march4 antibody

Background: MARCH4 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Alias Symbols: MARCH-IV, MGC104908, RNF174
Protein Interaction Partner: UBE2H, UBE2D1, ULBP1, CD4,
Protein Size: 410

Molecular Weight: 45 kDa

Gene ID: 57574

NCBI Accession: NM_020814, NP_065865

UniProt: Q9P2E8