Annexin IV antibody (N-Term)

Catalog No. ABIN2774909

Product Details anti-Annexin IV Antibody

Target: Annexin IV (ANXA4) (Annexin A4 (ANXA4))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: GLGTDEDAII SVLAYRNTAQ RQEIRTAYKS TIGRDLIDDL KSELSGNFEQ

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ANXA4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ANXA4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 321 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Annexin IV antibody (ABIN2774909)

de la Cuesta, Alvarez-Llamas, Maroto, Donado, Zubiri, Posada, Padial, Pinto, Barderas, Vivanco: "A proteomic focus on the alterations occurring at the human atherosclerotic coronary intima." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 4, pp. M110.003517, (2011) (PubMed).

Staquicini, Cardó-Vila, Kolonin, Trepel, Edwards, Nunes, Sergeeva, Efstathiou, Sun, Almeida, Tu, Botz, Wallace, OConnell, Krajewski, Gershenwald, Molldrem, Flamm, Koivunen, Pentz, Dias-Neto, Setubal et al.: "Vascular ligand-receptor mapping by direct combinatorial selection in cancer patients. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 46, pp. 18637-42, (2011) (PubMed).

Target Details for Annexin IV

Target: Annexin IV (ANXA4) (Annexin A4 (ANXA4))

Alternative Name: ANXA4 (ANXA4 Products)

Background: Annexin A4 (ANXA4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANXA4 has 45 to 59 % identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANXA4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANXA4 is almost exclusively expressed in epithelial cells.
Alias Symbols: ANX4, PIG28, ZAP36
Protein Interaction Partner: PADI4, UBC, EZH2, DNAJB11, MPST, ANXA7, ANXA3, ANXA1, TINF2, POT1, SUMO2, NMT2, SMPD1, Cdk1, UCHL5, H2AFX, NFKB1, GP2,
Protein Size: 321

Molecular Weight: 35 kDa

Gene ID: 307

NCBI Accession: NM_001153, NP_001144

UniProt: Q6LES2