EIF4A3 antibody (Middle Region)

Catalog No. ABIN2775226

Product Details anti-EIF4A3 Antibody

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QCHACIGGTN VGEDIRKLDY GQHVVAGTPG RVFDMIRRRS LRTRAIKMLV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against DDX48. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DDX48

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 411 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF4A3

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Alternative Name: DDX48 (EIF4A3 Products)

Synonyms: DDX48 antibody, NMP265 antibody, NUK34 antibody, eIF4AIII antibody, 2400003O03Rik antibody, Ddx48 antibody, eIF4A-III antibody, mKIAA0111 antibody, ddx48 antibody, nuk34 antibody, nmp265 antibody, eif4aiii antibody, EIF4A3 antibody, eif4a3 antibody, zgc:56139 antibody, XeIF-4AIII antibody, eif4a3-B antibody, eukaryotic translation initiation factor 4A3 antibody, eukaryotic initiation factor 4A-III antibody, eukaryotic translation initiation factor 4A3 S homeolog antibody, Eukaryotic initiation factor 4A-III antibody, EIF4A3 antibody, Eif4a3 antibody, eif4a3 antibody, LOC100185906 antibody, eif4a3.S antibody, if4a3 antibody

Background: DDX48 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
Alias Symbols: DDX48, NUK34, NMP265, eIF4AIII
Protein Interaction Partner: CDCA7L, PDCD4, HUWE1, POLDIP3, HAUS1, SUMO2, SUMO3, STAU1, UBC, LIN28B, SUMO1, WWOX, EED, RNF2, BMI1, SUZ12, ANP32A, PKM, NPLOC4, IPO11, PDLIM5, PLIN3, SRP14, RAD23B, AARS, FBXO6, SRPK2, SRPK1, UPF1, SMG1, RBM8A, ITGA4, CWC22, EEF2K, VCAM1, FN1, TRA2B, HN
Protein Size: 411

Molecular Weight: 45 kDa

Gene ID: 9775

NCBI Accession: NM_014740, NP_055555

UniProt: P38919