HDAC9 antibody (C-Term)

Catalog No. ABIN2775576

Product Details anti-HDAC9 Antibody

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Guinea Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Sequence: QVGAVKVKEE PVDSDEDAQI QEMESGEQAA FMQQVIGKDL APGFVIKVII

Predicted Reactivity: Guinea Pig: 100%, Human: 100%, Mouse: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against HDAC9. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HDAC9

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 590 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HDAC9 antibody (ABIN2775576)

Spallotta, Tardivo, Nanni, Rosati, Straino, Mai, Vecellio, Valente, Capogrossi, Farsetti, Martone, Bozzoni, Pontecorvi, Gaetano, Colussi: "Detrimental effect of class-selective histone deacetylase inhibitors during tissue regeneration following hindlimb ischemia." in: The Journal of biological chemistry, Vol. 288, Issue 32, pp. 22915-29, (2013) (PubMed).

Milde, Oehme, Korshunov, Kopp-Schneider, Remke, Northcott, Deubzer, Lodrini, Taylor, von Deimling, Pfister, Witt: "HDAC5 and HDAC9 in medulloblastoma: novel markers for risk stratification and role in tumor cell growth." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 12, pp. 3240-52, (2010) (PubMed).

Hang, Yang, Han, Cheng, Shang, Ashley, Zhou, Chang: "Chromatin regulation by Brg1 underlies heart muscle development and disease." in: Nature, Vol. 466, Issue 7302, pp. 62-7, (2010) (PubMed).

Target Details for HDAC9

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Alternative Name: HDAC9 (HDAC9 Products)

Synonyms: HD7 antibody, HD7b antibody, HD9 antibody, HDAC antibody, HDAC7 antibody, HDAC7B antibody, HDAC9B antibody, HDAC9FL antibody, HDRP antibody, MITR antibody, AV022454 antibody, D030072B18Rik antibody, HD7B antibody, Hdac7b antibody, Mitr antibody, mKIAA0744 antibody, hdac9 antibody, zgc:73392 antibody, TWIST antibody, HDAC9 antibody, DKFZp459E171 antibody, HDA09 antibody, HISTONE DEACETYLASE antibody, histone deacetylase 9 antibody, RGD1310748 antibody, RGD1563092 antibody, hdac9-A antibody, histone deacetylase 9 antibody, histone deacetylase 9b antibody, histone deacetylase 9 S homeolog antibody, HDAC9 antibody, Hdac9 antibody, hdac9b antibody, HDA9 antibody, hdac9.S antibody

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC9 has sequence homology to members of the histone deacetylase family. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. HDAC9 may play a role in hematopoiesis.Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
Alias Symbols: DKFZp779K1053, HD7, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, KIAA0744, MITR, HD9, HD7b
Protein Interaction Partner: NOP2, MKI67, EIF6, RCC1, ADAR, TOP1, TMPO, FIP1L1, CCAR2, TPX2, RBM14, ZC3H11A, HDAC9, NUP93, SMC3, H1FX, TPR, HINT1, CTBP1, BCL6, Zfp521, TRIM29, TP53, MAML1, SMARCA4, PARP1, CEBPB, H3F3A, RL2, CAMK1, YWHAE, HDAC1, SFN, MEF2B, ANKRA2, RFXANK, PKD1, SYK,
Protein Size: 590

Molecular Weight: 65 kDa

Gene ID: 9734

NCBI Accession: NM_014707, NP_055522

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development