MARCH2 antibody (Middle Region)

Catalog No. ABIN2775654

Product Details anti-MARCH2 Antibody

Target: MARCH2 (Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Guinea Pig, Rabbit, Dog, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LFITPLAAIS GWLCLRGAQD HLRLHSQLEA VGLIALTIAL FTIYVLWTLV

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MARCH2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MARCH2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 246 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MARCH2 antibody (ABIN2775654)

Wakisaka, Tsuda, Yamada, Yagihara, Kitamura: "Long-term results of saphenous vein graft for coronary stenosis caused by Kawasaki disease." in: Circulation journal : official journal of the Japanese Circulation Society, Vol. 73, Issue 1, pp. 73-7, (2008) (PubMed).

Target Details for MARCH2

Target: MARCH2 (Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2))

Alternative Name: MARCH2 (MARCH2 Products)

Synonyms: MARCH-II antibody, RNF172 antibody, rgd1306395 antibody, marh2 antibody, RGD1306395 antibody, 9530046H09Rik antibody, membrane associated ring-CH-type finger 2 antibody, membrane associated ring-CH-type finger 2 S homeolog antibody, E3 ubiquitin-protein ligase MARCH2 antibody, membrane-associated ring finger (C3HC4) 2 antibody, MARCH2 antibody, march2.S antibody, march2 antibody, Tsp_09446 antibody, marh2 antibody, March2 antibody

Background: MARCH2 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH2 may be involved in endosomal trafficking through interaction with STX6.
Alias Symbols: HSPC240, MARCH-II, RNF172
Protein Interaction Partner: LDLRAD1, SYNE4, CCDC155, FATE1, UBE2H, UBE2D1, CDC34, GOPC, STX6, CFTR, ADRB2, ELAVL1, DLG1,
Protein Size: 246

Molecular Weight: 27 kDa

Gene ID: 51257

NCBI Accession: NM_001005415, NP_001005415

UniProt: Q9P0N8