CIAO1 antibody (Middle Region)

Catalog No. ABIN2775776

Product Details anti-CIAO1 Antibody

Target: CIAO1 (Cytosolic Iron-Sulfur Protein Assembly 1 (CIAO1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIAO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: SVLNSHTQDV KHVVWHPSQE LLASASYDDT VKLYREEEDD WVCCATLEGH

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%

Characteristics: This is a rabbit polyclonal antibody against CIAO1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CIAO1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 339 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CIAO1 antibody (ABIN2775776)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for CIAO1

Target: CIAO1 (Cytosolic Iron-Sulfur Protein Assembly 1 (CIAO1))

Alternative Name: CIAO1 (CIAO1 Products)

Synonyms: CG12797 antibody, Dmel\\CG12797 antibody, DDBDRAFT_0190506 antibody, DDBDRAFT_0266672 antibody, DDB_0190506 antibody, DDB_0266672 antibody, CIA1 antibody, WDR39 antibody, AW210570 antibody, Wdr39 antibody, wdr39 antibody, zgc:55911 antibody, zgc:77394 antibody, CG12797 gene product from transcript CG12797-RA antibody, WD40 protein Ciao1 antibody, CIAO1 antibody, WD40 repeat-containing protein antibody, cytosolic iron-sulfur assembly component 1 antibody, cytosolic iron-sulfur protein assembly 1 antibody, Ciao1 antibody, cgd1_2230 antibody, CC1G_05316 antibody, ciao1 antibody, CIAO1 antibody

Background: CIAO1 belongs to the WD repeat CIA1 family. It seems to specifically modulate the transactivation activity of WT1.
Alias Symbols: CIA1, WDR39
Protein Interaction Partner: FAM96B, SF1, MEOX1, FAM96A, MMS19, FMNL2, TOE1, DPP9, FMNL3, CTU1, PPIL4, MAK16, WDR75, NSRP1, EEPD1, WDR61, CDC73, UPF3B, NARFL, YAE1D1, TYW1, ELP2, ELP3, UCKL1, CDKAL1, DPP8, ELP6, PAF1, PLEKHA5, PHAX, RTEL1, ELP4, POLA2, ELP5, WDR43, ERP29, GLRX3, CTR9
Protein Size: 339

Molecular Weight: 38 kDa

Gene ID: 9391

NCBI Accession: NM_004804, NP_004795

UniProt: O76071