TRIM27 antibody (Middle Region)

Catalog No. ABIN2775961

Product Details anti-TRIM27 Antibody

Target: TRIM27 (Tripartite Motif Containing 27 (TRIM27))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM27 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KRVKDLKKRR RAQGEQARAE LLSLTQMERE KIVWEFEQLY HSLKEHEYRL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TRIM27. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRIM27

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 358 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TRIM27

Target: TRIM27 (Tripartite Motif Containing 27 (TRIM27))

Alternative Name: TRIM27 (TRIM27 Products)

Synonyms: RFP antibody, RNF76 antibody, AW538890 antibody, Gm19403 antibody, Rfp antibody, TRIM27 antibody, Rfp1 antibody, tripartite motif containing 27 antibody, tripartite motif-containing 27 antibody, TRIM27 antibody, Trim27 antibody

Background: TRIM27 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.
Alias Symbols: RFP, RNF76
Protein Interaction Partner: LGALS14, FAM214A, USE1, CCDC94, ZNF446, TBC1D22B, ABCF3, RBM41, CCDC87, C14orf105, RBM23, WDYHV1, CHCHD3, KCTD9, FAM193B, CCHCR1, FAM64A, FBXW5, ZNF581, AMOTL2, WT1-AS, SLC15A3, CDKL3, VPS28, MEMO1, TPRKB, ARHGEF3, NME7, BABAM1, MAT2B, INPP5J, FOXB1, CDK1
Protein Size: 358

Molecular Weight: 41 kDa

Gene ID: 5987

NCBI Accession: NM_030950, NP_112212

UniProt: P14373