CREM antibody (N-Term)

Catalog No. ABIN2776016

Product Details anti-CREM Antibody

Target: CREM (CAMP Responsive Element Modulator (CREM))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Sheep, Guinea Pig, Horse, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREM antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MAAATGDMPT YQIRAPTAAL PQGVVMAASP GSLHSPQQLA EEATRKRELR

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against CREM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CREM

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 120 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CREM antibody (ABIN2776016)

Sunahori, Juang, Tsokos: "Methylation status of CpG islands flanking a cAMP response element motif on the protein phosphatase 2Ac alpha promoter determines CREB binding and activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 3, pp. 1500-8, (2009) (PubMed).

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for CREM

Target: CREM (CAMP Responsive Element Modulator (CREM))

Alternative Name: CREM (CREM Products)

Synonyms: CREM antibody, ICER antibody, CREM-2 antibody, hCREM-2 antibody, ICERI antibody, Icer antibody, cAMP responsive element modulator antibody, CREM antibody, Crem antibody

Background: CREM is a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription.
Alias Symbols: ICER, CREM-2, hCREM-2
Protein Interaction Partner: CREB1, PIAS3, CASP8AP2, UBE2I, UBC, SP100, ATXN1, EMP3, HHV8GK18_gp81, MAPK3, CREB3L4, HDAC1, CREB3L1, TAF4, CAMK2D, GSK3A, FHL5, GSK3B, KCNIP3, CAMK2G, PRKACA, CSNK1A1, CREBBP, TBP, PRKCA, SPI1, CREM, ATF1, CDC34, CDK1, CSNK2A2, CSNK2A1, CD34,
Protein Size: 120

Molecular Weight: 13 kDa

Gene ID: 1390

NCBI Accession: NM_182717, NP_874386

UniProt: O75519

Pathways: Retinoic Acid Receptor Signaling Pathway