CHRNA4 antibody (N-Term)

Catalog No. ABIN2776101

Product Details anti-CHRNA4 Antibody

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AEERLLKKLF SGYNKWSRPV ANISDVVLVR FGLSIAQLID VDEKNQMMTT

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CHRNA4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHRNA4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 627 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHRNA4 antibody (ABIN2776101)

Ishizuka, Ozawa, Goshima, Watanabe: "Involvement of nicotinic acetylcholine receptor in the proliferation of mouse induced pluripotent stem cells." in: Life sciences, Vol. 90, Issue 17-18, pp. 637-48, (2012) (PubMed).

Target Details for CHRNA4

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Alternative Name: CHRNA4 (CHRNA4 Products)

Synonyms: NACHRA4 antibody, BFNC antibody, EBN antibody, EBN1 antibody, NACHR antibody, NACRA4 antibody, NARAC antibody, Acra-4 antibody, Acra4 antibody, ENFL1 antibody, neuronal acetylcholine receptor subunit alpha-4 antibody, neuronal acetylcholine receptor subunit alpha-4-like antibody, cholinergic receptor nicotinic alpha 4 subunit antibody, cholinergic receptor, nicotinic, alpha 4b antibody, cholinergic receptor, nicotinic, alpha polypeptide 4 antibody, CpipJ_CPIJ002434 antibody, LOC100368057 antibody, CHRNA4 antibody, chrna4b antibody, Chrna4 antibody

Background: CHRNA4 is a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: BFNC, EBN, EBN1, NACRA4, NACHR, NACHRA4
Protein Interaction Partner: Ubqln1, CSNK2B, VSNL1, CHRNB4, CHRNB2, YWHAH, CRELD2,
Protein Size: 627

Molecular Weight: 67 kDa

Gene ID: 1137

NCBI Accession: NM_000744, NP_000735

UniProt: P43681

Pathways: Synaptic Membrane