EED antibody (N-Term)

Catalog No. ABIN2776406

Product Details anti-EED Antibody

Target: EED (Embryonic Ectoderm Development (EED))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AKKQKLSSDE NSNPDLSGDE NDDAVSIESG TNTERPDTPT NTPNAPGRKS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against Eed. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 441 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: Eed (EED Products)

Synonyms: Xeed antibody, heed antibody, wait1 antibody, EED antibody, HEED antibody, WAIT1 antibody, zgc:112509 antibody, Xeed-B antibody, eed antibody, psXEED antibody, ENSMUSG00000039373 antibody, l(7)5Rn antibody, l7Rn5 antibody, lusk antibody, Xeed-A antibody, embryonic ectoderm development antibody, embryonic ectoderm development S homeolog antibody, microRNA 6755 antibody, embryonic ectoderm development L homeolog antibody, eed antibody, EED antibody, eed.S antibody, MIR6755 antibody, Eed antibody, eed.L antibody

Background: Eed is a polycomb group (PcG) protein. It is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Eed plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Eed is required for stable maintenance of XCI in both embryonic and extra-embryonic tissues. Eed may prevent transcriptional activation of facultative heterochromatin during differentiation. Eed is required for development of secondary trophoblast giant cells during placental development. Eed may regulate hippocampal synaptic plasticity in the developing brain.
Alias Symbols: ENSMUSG00000039373, l(7)5Rn, l7Rn5, lusk
Protein Interaction Partner: Ezh2, Suz12, Rab3gap1, Ddx47, Nop10, Nip7, Bola2, Tmem14c, Uqcr10, Eif3f, Cbwd1, Smc5, Etf1, Svil, Fbxo11, Srsf7, Safb, Safb2, Abcf1, Bag6, C330027C09Rik, Pak2, Pphln1, Ttll12, Sun2, Tomm22, Eif3l, E430025E21Rik, Eny2, Dcaf13, Trio, Ccar2, Fermt2, Vps41,
Protein Size: 441

Molecular Weight: 50 kDa

Gene ID: 13626

NCBI Accession: NM_021876, NP_068676

UniProt: Q921E6