FUBP1 antibody (Middle Region)

Catalog No. ABIN2776409

Product Details anti-FUBP1 Antibody

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FUBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: YYAHYYQQQA QPPPAAPAGA PTTTQTNGQG DQQNPAPAGQ VDYTKAWEEY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against FUBP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FUBP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 644 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FUBP1 antibody (ABIN2776409)

Milosevic, Bulau, Mortz, Eickelberg: "Subcellular fractionation of TGF-beta1-stimulated lung epithelial cells: a novel proteomic approach for identifying signaling intermediates." in: Proteomics, Vol. 9, Issue 5, pp. 1230-40, (2009) (PubMed).

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for FUBP1

Target: FUBP1 (Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))

Alternative Name: FUBP1 (FUBP1 Products)

Synonyms: FBP antibody, FUBP antibody, fbp antibody, fubp antibody, MGC53183 antibody, MGC75632 antibody, FUBP1 antibody, DKFZp468N0812 antibody, 9530027K12Rik antibody, D3Ertd330e antibody, Fubp antibody, Fubp4 antibody, MGC78835 antibody, wu:fa92h11 antibody, wu:fb24h11 antibody, wu:fc26f06 antibody, zgc:66046 antibody, far upstream element binding protein 1 antibody, far upstream element (FUSE) binding protein 1 S homeolog antibody, far upstream element (FUSE) binding protein 1 antibody, far upstream element binding protein 1 L homeolog antibody, FUBP1 antibody, fubp1.S antibody, fubp1 antibody, Fubp1 antibody, fubp1.L antibody

Background: FUBP1 is a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FBP, FUBP
Protein Interaction Partner: UBC, MDM2, EED, DDX19A, NSUN2, PLD3, ARPC2, ACTR2, ACTR3, ARPC4, HIST1H2BO, SH3GL1, RPA1, PPP5C, LGALS1, CNN2, BAG1, ACO1, AARSD1, GRPEL1, NIF3L1, RPRD1B, CD81, ITGA4, FN1, ATF2, AIMP2, VCAM1, DHX36, MRPL32, MRPL38, MRPL41, MRPL2, CERS2, MRPL42, MRPL46, P
Protein Size: 644

Molecular Weight: 67 kDa

Gene ID: 8880

NCBI Accession: NM_003902, NP_003893

UniProt: Q96AE4