UPF3B antibody (UPF3 Regulator of Nonsense Transcripts Homolog B (Yeast)) (Middle Region)

Details for Product anti-UPF3B Antibody No. ABIN2776615, Supplier: Log in to see
Antigen
  • HUPF3B
  • MRXS14
  • RENT3B
  • UPF3X
  • 5730594O13Rik
  • AI317193
  • AW541158
  • RGD1560264
  • UPF3 regulator of nonsense transcripts homolog B (yeast)
  • UPF3B
  • Upf3b
Epitope
Middle Region
3
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
21
6
6
4
2
2
1
1
Host
Rabbit
21
Clonality
Polyclonal
Application
Western Blotting (WB)
19
7
7
5
3
2
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human UPF3B
Blocking Peptide Blocking peptide for this product available: ABIN985946
Sequence KIDRIPERDK LKDEPKIKVH RFLLQAVNQK NLLKKPEKGD EKELDKREKA
Predicted Reactivity Dog: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against UPF3B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name UPF3B (UPF3B Antibody Abstract)
Background UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
Alias Symbols: HUPF3B, MRXS14, RENT3B, UPF3X
Protein Interaction Partner: NCBP1, CIAO1, STAU1, UBC, UPF1, HECW2, RBM8A, UPF2, SMG1, EIF4A3, WIBG, WBP11, RNPS1, SART1, TOP2A, SNRPA1, NXF1, CAND1, COPS5, CUL5, USP21, EXOSC4, MCRS1, XRN1, TTC19, EIF6, UPF3B, HBB, UPF3A,
Protein Size: 483
Molecular Weight 58 kDa
Gene ID 65109
NCBI Accession NM_080632, NP_542199
UniProt Q9BZI7
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 483 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-UPF3 Regulator of Nonsense Transcripts Homolog B (Yeast) (UPF3B) (Middle Region) antibody (ABIN2776615) anti-UPF3 Regulator of Nonsense Transcripts Homolog B (Yeast) (UPF3B) (Middle Region) antibody
Background publications Chamieh, Ballut, Bonneau, Le Hir: "NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity." in: Nature structural & molecular biology, Vol. 15, Issue 1, pp. 85-93, 2008 (PubMed).

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