Keratin 10 antibody (N-Term)

Catalog No. ABIN2776960

Product Details anti-Keratin 10 Antibody

Target: Keratin 10 (KRT10)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EKVTMQNLND RLASYLDKVR ALEESNYELE GKIKEWYEKH GNSHQGEPRD

Predicted Reactivity: Cow: 93%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 86%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against KRT10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KRT10

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 584 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Keratin 10 antibody (ABIN2776960)

Tsubota, Akiyama, Kanitakis, Sakai, Nomura, Claudy, Shimizu: "Mild recessive bullous congenital ichthyosiform erythroderma due to a previously unidentified homozygous keratin 10 nonsense mutation." in: The Journal of investigative dermatology, Vol. 128, Issue 7, pp. 1648-52, (2008) (PubMed).

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: KRT10 (KRT10 Products)

Synonyms: BCIE antibody, BIE antibody, CK10 antibody, EHK antibody, K10 antibody, KPP antibody, D130054E02Rik antibody, K1C1 antibody, Krt-1.10 antibody, Krt1-10 antibody, Ka10 antibody, Ker10 antibody, KRT10 antibody, KRT25 antibody, KRT27 antibody, KRT28 antibody, keratin 10 antibody, keratin 10, type I S homeolog antibody, keratin, type I cytoskeletal 25 antibody, KRT10 antibody, Krt10 antibody, krt10.S antibody, LOC101085587 antibody

Background: KRT10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in the gene encoding KRT10 are associated with epidermolytic hyperkeratosis. This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CK10, K10, KPP, BIE, EHK, BCIE
Protein Interaction Partner: UBC, Fbxl16, ADRB2, PAN2, Nphp4, Iqcb1, Cep290, Nphp1, Invs, VCAM1, SRC, ITGA4, FN1, ALB, MAGOH, TRAF3IP1, EIF4A3, AHNAK2, KRT2, KRT1, ELAVL1, UBASH3B, SHC1, PIK3R2, INPPL1, GRB2, EPS15, CRK, AP2M1, CBL, NEDD8, CAND1, DCUN1D1, COPS5, COPS6, CUL1, CUL2, CU
Protein Size: 584

Molecular Weight: 59 kDa

Gene ID: 3858

NCBI Accession: NM_000421, NP_000412

UniProt: P13645