FGA antibody (Middle Region)

Catalog No. ABIN2776978

Product Details anti-FGA Antibody

Target: FGA (Fibrinogen alpha Chain (FGA))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGA antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SSSYSKQFTS STSYNRGDST FESKSYKMAD EAGSEADHEG THSTKRGHAK

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against FGA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FGA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 644 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FGA antibody (ABIN2776978)

Matsumoto, Matsuura, Aoki, Maehashi, Iwamoto, Ohkawa, Yoshida, Yanaga, Takada: "An efficient system for secretory production of fibrinogen using a hepatocellular carcinoma cell line." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 45, Issue 3, pp. 315-25, (2016) (PubMed).

Prieto-García, Zheng, Adachi, Xing, Lane, Chung, Anderson, Hansbro, Castells, Stevens: "Mast cell restricted mouse and human tryptase·heparin complexes hinder thrombin-induced coagulation of plasma and the generation of fibrin by proteolytically destroying fibrinogen." in: The Journal of biological chemistry, Vol. 287, Issue 11, pp. 7834-44, (2012) (PubMed).

Target Details for FGA

Target: FGA (Fibrinogen alpha Chain (FGA))

Alternative Name: FGA (FGA Products)

Synonyms: Fib2 antibody, ENSMUSG00000059807 antibody, Fib antibody, Ac1873 antibody, Fba5e antibody, fibrinogen alpha chain antibody, FGA antibody, Fga antibody, LOC698244 antibody

Background: FGA is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in its gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus.
Alias Symbols: Fib2, MGC119422, MGC119423, MGC119425
Protein Interaction Partner: ASB7, ASB6, FN1, APOA1, ALB, UBC, SUMO2, MIS12, KHDRBS2, ITGA2B, SERPINA5, CDH5, SERPINF2, THBS1, F2, ITGB3, PLAT, HRG, PRRC2A, FGG, FGB, FGA, F13A1, NID1,
Protein Size: 644

Molecular Weight: 66 kDa

Gene ID: 2243

NCBI Accession: NM_021871, NP_068657

UniProt: Q4QQH7