PPL antibody (Middle Region)

Catalog No. ABIN2777127

Product Details anti-PPL Antibody

Target: PPL (Periplakin (PPL))

Binding Specificity: Middle Region

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPL antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EKSRAQEKVT EKEVVKLQND PQLEAEYQQL QEDHQRQDQL REKQEEELSF

Predicted Reactivity: Human: 100%, Pig: 82%

Characteristics: This is a rabbit polyclonal antibody against PPL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PPL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1756 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPL

Target: PPL (Periplakin (PPL))

Alternative Name: PPL (PPL Products)

Synonyms: cb180 antibody, sb:cb180 antibody, im:7140067 antibody, im:7149519 antibody, AW553870 antibody, periplakin antibody, periplakin L homeolog antibody, PPL antibody, ppl antibody, ppl.L antibody, Ppl antibody

Background: PPL is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: KIAA0568, MGC134872
Protein Interaction Partner: CEP57L1, ZSCAN1, RAB3IP, TSC22D4, RALBP1, AHCYL1, VIM, TRAF2, TRIM27, ALAS1, AKT1, SKIL, UBC, USP7, CUL1, ITSN1, Cdk1, KAZN, PPHLN1, EVPL, CASP6, OPRM1, COL17A1, KRT8, EZR,
Protein Size: 1756

Molecular Weight: 205 kDa

Gene ID: 5493

NCBI Accession: NM_002705, NP_002696

UniProt: O60437