YAF2 antibody (Middle Region)

Catalog No. ABIN2777311

Product Details anti-YAF2 Antibody

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: KGTSTRKPRP VSQLVAQQVT QQFVPPTQSK KEKKDKVEKE KSEKETTSKK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against YAF2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human YAF2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 180 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for YAF2

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Alternative Name: YAF2 (YAF2 Products)

Synonyms: MGC75686 antibody, YAF2 antibody, zgc:136577 antibody, si:ch211-155a11.5 antibody, 2810021M11Rik antibody, YY1 associated factor 2 antibody, YY1-associated factor 2 antibody, yaf2 antibody, YAF2 antibody, LOC100221270 antibody, Yaf2 antibody

Background: YAF2 interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. YAF2 contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1.The protein encoded by this gene interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two have been confirmed to date.
Alias Symbols: MGC41856
Protein Interaction Partner: SETBP1, XAGE1E, RING1, RNF2, BMI1, GABPB1, CSNK2A2, UBC, CAND1, PCGF1, KDM2B, PCGF5, PCGF6, L3MBTL2, FBRS, BCORL1, BCOR, MGA, CBX3, WDR5, PCGF3, YAF2, USP7, PCGF2, TFDP1, SKP1, HDAC2, E2F6, CSNK2A1, ZFP42, YY1, MYCN,
Protein Size: 180

Molecular Weight: 20 kDa

Gene ID: 10138

NCBI Accession: NM_005748, NP_005739

UniProt: Q8IY57