AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (Middle Region)

Details for Product anti-AKAP8 Antibody No. ABIN2777315, Supplier: Log in to see
Antigen
  • MGC108502
  • AKAP8
  • AKAP 95
  • AKAP-8
  • AKAP-95
  • AKAP95
  • 1200016A02Rik
  • AA673585
  • AU015639
  • Akap95
  • A kinase (PRKA) anchor protein 8
  • AKAP8
  • akap8
  • Akap8
Epitope
Middle Region
12
8
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Pig (Porcine), Rat (Rattus)
90
41
37
11
5
3
2
2
1
1
1
Host
Rabbit
58
17
16
Clonality
Polyclonal
Conjugate
This AKAP8 antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
73
28
27
19
16
13
7
7
4
3
2
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human AKAP8
Blocking Peptide Blocking peptide for this product available: ABIN973061
Sequence DVLAEVITAA VRAVDGEGAP APESSGEPAE DEGPTDTAEA GSDPQAEQLL
Predicted Reactivity Cow: 93%, Dog: 100%, Horse: 100%, Human: 100%, Pig: 86%, Rat: 86%
Characteristics This is a rabbit polyclonal antibody against AKAP8. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name AKAP8 (AKAP8 Antibody Abstract)
Background The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. AKAP8 is a member of the AKAP family. It is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: AKAP95, DKFZp586B1222, AKAP-8, AKAP 95, AKAP-95
Protein Interaction Partner: SUMO2, UBC, RPA3, RPA2, RPA1, BMI1, RNF2, EZH2, HIPK4, HDAC11, CSNK2A1, MYC, ESR1, BARD1, LRRK2, CAND1, COPS5, CUL3, HNRNPA1, ELAVL1, Mis12, Aurkb, TADA2A, CASP3, DDX5, PRKAR2A, CCND1, RELA, CCND3, CCND2, MCM2, AMY1C, AMY1A,
Protein Size: 692
Molecular Weight 76 kDa
Gene ID 10270
NCBI Accession NM_005858, NP_005849
UniProt O43823
Research Area Kinases/Phosphatases, Signaling
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 692 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (Middle Region) (ABIN2777315) WB Suggested Anti-AKAP8 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positiv...
Background publications Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).