Claudin 1 antibody (C-Term)

Catalog No. ABIN2778045

Product Details anti-Claudin 1 Antibody

Target: Claudin 1 (CLDN1)

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Dog, Cow, Sheep, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: GQALFTGWAA ASLCLLGGAL LCCSCPRKTT SYPTPRPYPK PAPSSGKDYV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against CLDN1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CLDN1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 211 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Claudin 1 antibody (ABIN2778045)

Coyne, Gambling, Boucher, Carson, Johnson: "Role of claudin interactions in airway tight junctional permeability." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 285, Issue 5, pp. L1166-78, (2003) (PubMed).

Target Details for Claudin 1

Target: Claudin 1 (CLDN1)

Alternative Name: CLDN1 (CLDN1 Products)

Background: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This protein, a member of the claudin family, is an integral membrane protein and a component of tight junction strands.
Alias Symbols: CLD1, SEMP1, ILVASC
Protein Interaction Partner: UBC, DRG1, LNX1, TJP3, BRD4, MPDZ, TJP2, CLDN1, INADL, CLDN3, WNK4, TJP1, MMP14, MMP2, CLDN5,
Protein Size: 211

Molecular Weight: 23 kDa

Gene ID: 9076

NCBI Accession: NM_021101, NP_066924

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C