Afadin antibody (N-Term)

Catalog No. ABIN2778340

Product Details anti-Afadin Antibody

Target: Afadin (MLLT4)

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Afadin antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GVIQNFKRTL SKKEKKEKKK REKEALRQAS DKDDRPFQGE DVENSRLAAE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 85%, Rat: 100%, Yeast: 92%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against MLLT4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MLLT4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 674 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Afadin

Target: Afadin (MLLT4)

Alternative Name: MLLT4 (MLLT4 Products)

Synonyms: MLLT4 antibody, af-6 antibody, af6 antibody, si:ch211-278j12.1 antibody, AF6 antibody, RP3-431P23.3 antibody, 5033403D15Rik antibody, Af-6 antibody, Afadin antibody, Gm314 antibody, I-afadin antibody, S-afadin antibody, Af6 antibody, afadin, adherens junction formation factor antibody, afadin, adherens junction formation factor L homeolog antibody, afadin, adherens junction formation factor a antibody, afadin antibody, AFDN antibody, afdn.L antibody, afdna antibody, LOC589923 antibody, LOC100177180 antibody, afdn antibody, Afdn antibody

Background: AF6 is a Ras target that regulates cell-cell adhesions downstream of Ras activation. It is fused with MLL in leukemias caused by t(6,11) translocations.
Alias Symbols: RP3-470B24.4, AF-6, AF6, AFADIN, FLJ34371, RP3-431P23.3
Protein Interaction Partner: UBC, LGR4, LATS2, YWHAQ, YWHAE, VCAM1, SMAD2, ITGA4, GRB2, FN1, SIRT7, HNRNPA1, ARRB2, YWHAG, YWHAB, KMT2A, MEN1, PVRL4, PICK1, RIT2, SORBS2, F11R, NRXN2, SSX2IP, RIT1, NRXN3, PVRL3, PVRL1, MRAS, SORBS1, EPHB6, EPHA7, JAG1, EPHB3, RALGDS, TJP1, EPHB2, PVR
Protein Size: 674

Molecular Weight: 74 kDa

Gene ID: 4301

NCBI Accession: NM_001040000

UniProt: P55196

Pathways: Cell-Cell Junction Organization