AIF antibody (C-Term)

Catalog No. ABIN2778665

Product Details anti-AIF Antibody

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Horse, Sheep, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: VDSSLPTVGV FAKATAQDNP KSATEQSGTG IRSESETESE ASEITIPPST

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against PDCD8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PDCD8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 326 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: PDCD8 (AIFM1 Products)

Synonyms: AIF antibody, CMTX4 antibody, COWCK antibody, COXPD6 antibody, PDCD8 antibody, CG7263 antibody, DmAIF antibody, Dmel\\CG7263 antibody, GB16024 antibody, DDBDRAFT_0187853 antibody, DDBDRAFT_0191137 antibody, DDB_0187853 antibody, DDB_0191137 antibody, aif antibody, pdcd8 antibody, AIFM1 antibody, PCD8 antibody, AIFsh2 antibody, Hq antibody, Pdcd8 antibody, mAIF antibody, Aif antibody, zgc:91994 antibody, apoptosis inducing factor mitochondria associated 1 antibody, allograft inflammatory factor 1 antibody, Apoptosis inducing factor antibody, apoptosis-inducing factor 1, mitochondrial antibody, apoptosis inducing factor antibody, apoptosis inducing factor, mitochondria associated 1 antibody, apoptosis-inducing factor, mitochondrion-associated, 1 antibody, apoptosis-inducing factor, mitochondrion-associated 1 antibody, AIFM1 antibody, AIF1 antibody, AIF antibody, LOC412212 antibody, aif antibody, aifm1 antibody, Aifm1 antibody

Background: AIFM1 (PDCD8) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, AIFM1 induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene.
Alias Symbols: AIF, PDCD8, COXPD6
Protein Interaction Partner: ISG15, STAU1, BAG6, UBC, NEDD8, MDM2, ASB12, RNF2, BMI1, SUZ12, PARK2, BAG3, ADRB2, HDAC11, OXSR1, ILK, PAN2, NOS2, MLH1, CFTR, ESR1, TST, FSCN1, CPOX, SHC1, FBXO6, TSC22D4, CAND1, COPS5, CUL1, CUL2, CUL3, CUL5, TONSL, BLNK, PIDD1, XIAP, AMFR, Tubb4b, Tub
Protein Size: 326

Molecular Weight: 36 kDa

Gene ID: 9131

NCBI Accession: NM_145813, NP_665812

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect