CARS antibody (N-Term)

Catalog No. ABIN2778729

Product Details anti-CARS Antibody

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Binding Specificity: N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MQTPPLQQPH QEQVFLAFLV IVIPSFLTKE VFIPQDGKKV TWYCCGPTVY

Predicted Reactivity: Human: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CARS. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CARS

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 738 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CARS antibody (ABIN2778729)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for CARS

Target: CARS (Cysteinyl-tRNA Synthetase (CARS))

Alternative Name: CARS (CARS Products)

Synonyms: sb:cb71 antibody, CARS antibody, BcDNA:LD21177 antibody, CG8431 antibody, CRS antibody, CysRS antibody, Dmel\\CG8431 antibody, BA0089 antibody, DDBDRAFT_0215191 antibody, DDBDRAFT_0231320 antibody, DDB_0215191 antibody, DDB_0231320 antibody, DDBDRAFT_0187313 antibody, DDBDRAFT_0231318 antibody, DDB_0187313 antibody, DDB_0231318 antibody, T16B12.16 antibody, K15E6.3 antibody, K15E6_3 antibody, CARS1 antibody, CYSRS antibody, MGC:11246 antibody, CA3 antibody, cysteinyl-tRNA synthetase antibody, Cysteinyl-tRNA synthetase antibody, cysteine--tRNA ligase antibody, cysteine-tRNA ligase antibody, Cysteinyl-tRNA synthetase, class Ia family protein antibody, cysteinyl-tRNA synthetase CysS antibody, cysteinyl-tRNA synthetase S homeolog antibody, CARS antibody, cars antibody, CysRS antibody, cysS antibody, APH_RS02395 antibody, mcysS antibody, SYCO ARATH antibody, AT3G56300 antibody, AT5G38830 antibody, cars.S antibody, Cars antibody

Background: CARS is a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Alias Symbols: CARS1, CYSRS, MGC:11246
Protein Interaction Partner: UBC, DNM1L, CAND1, LUC7L2, THUMPD3, JMJD6, XPO7, FAF1, SEC23A, SEC24D, HIRIP3, HIST1H4A, TTC1, TROVE2, SHMT1, PPM1G, PPAT, PAWR, KARS, IDE, HSPA4, GFPT1, GART, GARS, APEH, APP, SUMO2, PACSIN1, PACSIN3, PACSIN2, PSTPIP1, EEF1G,
Protein Size: 738

Molecular Weight: 81 kDa

Gene ID: 833

NCBI Accession: NM_001014438, NP_001014438