PSMA1 antibody (N-Term)

Catalog No. ABIN2778811

Product Details anti-PSMA1 Antibody

Target: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Cow, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMA1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MFRNQYDNDV TVWSPQGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against PSMA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PSMA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 263 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PSMA1

Target: PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))

Alternative Name: PSMA1 (PSMA1 Products)

Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA1 is a member of the peptidase T1A family which is a 20S core alpha subunit.The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified.
Alias Symbols: NU, HC2, PROS30
Protein Interaction Partner: PRDM14, CEP70, CCDC102B, GNPTAB, CEP72, KCTD9, ROPN1, ABI3, SH3GLB1, APIP, LDOC1, MTUS2, MAPRE3, MAPRE1, IKZF3, MID2, EHMT2, PNMA2, IKZF1, TRIM10, HUWE1, HOMER3, PNMA1, KRT38, BLZF1, MAD1L1, MKRN3, REL, PSMA3, MLH1, KRT31, KRT15, KRTAP5-9, GOLGA2, CDA, CC
Protein Size: 263

Molecular Weight: 29 kDa

Gene ID: 5682

NCBI Accession: NM_002786, NP_002777

UniProt: P25786

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA