RPN1 antibody (Middle Region)

Catalog No. ABIN2778827

Product Details anti-RPN1 Antibody

Target: RPN1 (Ribophorin 1 (RPN1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPN1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AAEARMKVAC ITEQVLTLVN KRIGLYRHFD ETVNRYKQSR DISTLNSGKK

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against RPN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPN1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 607 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RPN1 antibody (ABIN2778827)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for RPN1

Target: RPN1 (Ribophorin 1 (RPN1))

Alternative Name: RPN1 (RPN1 Products)

Synonyms: OST1 antibody, RBPH1 antibody, AU018702 antibody, D6Wsu137e antibody, Rpn-1 antibody, RIBI antibody, wu:fa12h07 antibody, wu:fc68b07 antibody, wu:fc88a12 antibody, ost1 antibody, xrpn1 antibody, ribophorin I antibody, ribophorin I L homeolog antibody, PVX_119685 antibody, NAEGRDRAFT_78171 antibody, RPN1 antibody, Rpn1 antibody, rpn1 antibody, rpn1.L antibody

Background: This protein is a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DKFZp686B16177, OST1, RBPH1
Protein Interaction Partner: UBQLN1, UBC, SGTA, SUMO3, STAU1, LGR4, SUMO1, NEDD8, MDM2, ASB5, ASB11, BMI1, EED, RNF2, EGFR, ADRB2, CLN5, CLN3, AMFR, OST4, MMS19, env, UBL4A, WHSC1, VCAM1, FN1, ATF2, VCP, ESR1, ECT2, STT3B, SND1, SYNCRIP, TECR, VDAC2, SSR3, RPN2, RPSA, ILF3, SLC25A5,
Protein Size: 607

Molecular Weight: 66 kDa

Gene ID: 6184

NCBI Accession: NM_002950, NP_002941

UniProt: P04843