DROSHA antibody (Middle Region)

Catalog No. ABIN2779039

Product Details anti-DROSHA Antibody

Target: DROSHA (Drosha, Ribonuclease Type III (DROSHA))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DROSHA antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AAMDALEKYN FPQMAHQKRF IERKYRQELK EMRWEREHQE REPDETEDIK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RNASEN. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNASEN

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1374 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DROSHA

Target: DROSHA (Drosha, Ribonuclease Type III (DROSHA))

Alternative Name: RNASEN (DROSHA Products)

Synonyms: im:7150667 antibody, zgc:158612 antibody, ETOHI2 antibody, HSA242976 antibody, RANSE3L antibody, RN3 antibody, RNASE3L antibody, RNASEN antibody, 1110013A17Rik antibody, AI874853 antibody, Etohi2 antibody, Rn3 antibody, Rnasen antibody, RGD1307626 antibody, ribonuclease type III, nuclear antibody, Ribonuclease 3 antibody, ribonuclease 3 antibody, drosha ribonuclease III antibody, drosha, ribonuclease type III antibody, rnasen antibody, Thicy_0885 antibody, Sinme_0866 antibody, Isova_2087 antibody, Sphch_0770 antibody, DROSHA antibody, Drosha antibody

Background: RNASEN is a ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is also involved in pre-rRNA processing. RNASEN cleaves double-strand RNA and does not cleave single-strand RNA. RNASEN is involved in the formation of GW bodies.Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002 [PubMed 12191433]). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003 [PubMed 14508493]).[supplied by OMIM].
Alias Symbols: DROSHA, ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN
Protein Interaction Partner: DDX5, HNRNPUL2, KLHL12, YLPM1, ZCCHC8, DGCR8, SKIV2L2, U2SURP, KHDRBS1, CARM1, RBM14, PDCD6, THRAP3, PPFIA1, SRPK1, PKP2, MEN1, LGALS3BP, HNRNPAB, WWOX, BMI1, SRPK2, ZNF512B, UBC, CUL3, PSMA1, BCL6, SP1,
Protein Size: 1374

Molecular Weight: 159 kDa

Gene ID: 29102

NCBI Accession: NM_013235, NP_037367

UniProt: Q9NRR4

Pathways: Regulatory RNA Pathways