DAZAP1 antibody (Middle Region)

Catalog No. ABIN2779144

Product Details anti-DAZAP1 Antibody

Target: DAZAP1 (DAZ Associated Protein 1 (DAZAP1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Zebrafish (Danio rerio), Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAZAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: GKKVEVKRAE PRDSKSQAPG QPGASQWGSR VVPNAANGWA GQPPPTWQQG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against DAZAP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DAZAP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 401 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DAZAP1

Target: DAZAP1 (DAZ Associated Protein 1 (DAZAP1))

Alternative Name: DAZAP1 (DAZAP1 Products)

Synonyms: MGC89358 antibody, DAZAP1 antibody, 2410042M16Rik antibody, mPrrp antibody, DAZ associated protein 1 antibody, DAZ associated protein 1 L homeolog antibody, dazap1 antibody, DAZAP1 antibody, Dazap1 antibody, dazap1.L antibody

Background: In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. DAZAP1 is a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.
Alias Symbols: MGC19907
Protein Interaction Partner: UBC, WWOX, RPA3, RPA2, RPA1, SUZ12, RNF2, EZH2, BMI1, rev, ITCH, RAD52, VCAM1, ITGA4, FN1, BRCA1, CAND1, COPS5, CUL1, CUL2, CUL3, CUL4B, CUL5, NEDD8, ELAVL1, SUMO2, DAZ1, DAZL,
Protein Size: 401

Molecular Weight: 43 kDa

Gene ID: 26528

NCBI Accession: NM_018959, NP_061832

UniProt: Q96EP5