HSP27 antibody (C-Term)

Catalog No. ABIN2779293

Product Details anti-HSP27 Antibody

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: SLSPEGTLTV EAPMPKLATQ SNEITIPVTF ESRAQLGGPE AAKSDETAAK

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against HSPB1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HSPB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 205 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HSP27 antibody (ABIN2779293)

Sookoian, Castaño, Scian, San Martino, Pirola: "Heat Shock Protein 27 is down-regulated in Ballooned Hepatocytes of Patients with Nonalcoholic Steatohepatitis (NASH)." in: Scientific reports, Vol. 6, pp. 22528, (2016) (PubMed).

Narjoz, Marisa, Imbeaud, Paris, Delacroix, Beaune, De Waziers: "Genomic consequences of cytochrome P450 2C9 overexpression in human hepatoma cells." in: Chemical research in toxicology, Vol. 22, Issue 5, pp. 779-87, (2009) (PubMed).

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1 (HSPB1 Products)

Synonyms: CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

Background: HSPB1, like the other heat shock proteins, is part of a complex system of molecular chaperones in epidermal keratinocytes.
Alias Symbols: CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27
Protein Interaction Partner: PCSK9, HUWE1, UBC, GATA1, MAPKAPK2, AKT1, CRYAB, MAP3K7, SUMO2, HAUS1, CEP57, AARSD1, BAG3, IRS4, HSF1, LCE3A, TGFB1I1, SUMO1, NEDD8, MDM2, ASB6, ASB10, ASB17, WWOX, ASB4, ZBTB1, RPA3, RPA2, RPA1, EZH2, ESR1, SOX2, NPM1, PLG, CS, MAPKAPK3, INS, HSPB1, SGA
Protein Size: 205

Molecular Weight: 23 kDa

Gene ID: 3315

NCBI Accession: NM_001540, NP_001531

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling