p53 antibody (Tumor Protein P53) (N-Term)

Details for Product anti-TP53 Antibody No. ABIN2779317, Supplier: Log in to see
Antigen
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Alternatives
anti-Human p53 antibody for Immunofluorescence (fixed cells)
Epitope
N-Term
170
117
84
83
69
65
55
55
54
53
49
47
46
45
40
32
31
31
25
23
23
22
20
18
17
16
15
15
15
15
15
14
11
10
10
9
9
9
8
8
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6
5
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5
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5
5
5
5
5
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5
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4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
2329
514
418
186
126
94
85
81
34
22
19
18
16
16
15
13
6
4
4
4
4
3
3
2
2
2
1
1
Host
Rabbit
1321
1113
7
3
3
1
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
78
76
63
53
45
44
30
30
25
25
25
24
24
24
24
23
23
23
21
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Western Blotting (WB)
1856
746
719
714
614
596
424
340
300
179
72
41
32
31
18
16
15
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TP53
Blocking Peptide Blocking peptide for this product available: ABIN985382
Sequence EEPQSDPSVE PPLSQETFSD LWKLLPENNV LSPLPSQAMD DLMLSPDDIE
Predicted Reactivity Cow: 100%, Dog: 80%, Guinea Pig: 90%, Horse: 90%, Human: 100%, Pig: 100%, Rabbit: 100%, Rat: 90%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against TP53. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name TP53 (TP53 Antibody Abstract)
Background TP53 acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.
Alias Symbols: LFS1, TRP53, p53
Protein Size: 393
Molecular Weight 44 kDa
Gene ID 7157
NCBI Accession NM_000546, NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 393 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) anti-Tumor Protein P53 (TP53) (N-Term) antibody
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) Host: Rabbit
Target Name: TP53
Sample Tissue: Human Fetal Liver
...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) Host: Rabbit
Target Name: TP53
Sample Tissue: Human Adult Placenta
Background publications Boehme, Kulikov, Blattner: "p53 stabilization in response to DNA damage requires Akt/PKB and DNA-PK." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 22, pp. 7785-90, 2008 (PubMed).