ZEB1 antibody (N-Term)

Catalog No. ABIN2779631

Product Details anti-ZEB1 Antibody

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Sequence: KDDECESDAE NEQNHDPNVE EFLQQQDTAV IFPEAPEEDQ RQGTPEASGH

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ZEB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZEB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1124 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ZEB1 antibody (ABIN2779631)

Lobert, Graichen, Morris: "Coordinated regulation of ?-tubulin isotypes and epithelial-to-mesenchymal transition protein ZEB1 in breast cancer cells." in: Biochemistry, Vol. 52, Issue 32, pp. 5482-90, (2013) (PubMed).

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1 (ZEB1 Products)

Background: ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM].
Alias Symbols: AREB6, BZP, MGC133261, NIL-2-A, NIL-2A, TCF8, ZEB, ZFHEP, ZFHX1A, FECD6, NIL2A, PPCD3, DELTAEF1
Protein Interaction Partner: SUMO1, CDK6, GTF2A1, UBR4, SUMO2, SIRT1, SOX2, CTBP2, CTBP1, SMAD7, SMAD6, SMARCA4, SERPINH1, SMAD3, EP300, DRAP1, KAT5, SMAD2, SMAD1, CDH1,
Protein Size: 1124

Molecular Weight: 124 kDa

Gene ID: 6935

NCBI Accession: NM_030751, NP_110378

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation