APEX1 antibody (N-Term)

Catalog No. ABIN2779778

Product Details anti-APEX1 Antibody

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Guinea Pig, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: KGLDWVKEEA PDILCLQETK CSENKLPAEL QELPGLSHQY WSAPSDKEGY

Predicted Reactivity: Cow: 92%, Dog: 92%, Goat: 83%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against APEX1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human APEX1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 318 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1 (APEX1 Products)

Synonyms: apex1 antibody, MGC76280 antibody, MGC79645 antibody, MGC78928 antibody, APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, Apex antibody, REF-1 antibody, BAP1 antibody, apex1a antibody, hm:zehn1532 antibody, wu:fc13d11 antibody, zgc:66204 antibody, Ref-1 antibody, apurinic/apyrimidinic endodeoxyribonuclease 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, apurinic/apyrimidinic endonuclease 1 antibody, APEX1 antibody, apex1 antibody, apex1.L antibody, CNG03640 antibody, CNH01280 antibody, nth antibody, Spirs_4119 antibody, Palpr_0103 antibody, Apex1 antibody

Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells.
Alias Symbols: APE, APX, APE1, APEN, APEX, HAP1, REF1
Protein Interaction Partner: GADD45A, UBC, RPA3, RPA2, RPA1, rev, CCDC124, RIC8A, PABPC1, TWF2, XPOT, HNRNPUL1, ARIH2, PAPSS2, NAE1, PSMG1, TXNRD1, TXN, TCEB1, LGALS1, HNRNPK, CAPNS1, SRPK2, SRPK1, MUTYH, HMGA1, HMGA2, GZMK, GZMA, YBX1, EP300, ASCL2, STAT3, SNRPD1, SFPQ, APP, UBE2I,
Protein Size: 318

Molecular Weight: 35 kDa

Gene ID: 328

NCBI Accession: NM_080648, NP_542379

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus