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ELF1 antibody (N-Term)

ELF1 Reactivity: Human, Mouse, Rabbit, Rat, Horse, Dog WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2779831
  • Target See all ELF1 Antibodies
    ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
    Binding Specificity
    • 15
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 39
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rabbit, Rat, Horse, Dog
    Host
    • 35
    • 3
    • 1
    Rabbit
    Clonality
    • 38
    • 1
    Polyclonal
    Conjugate
    • 25
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ELF1 antibody is un-conjugated
    Application
    • 36
    • 13
    • 13
    • 12
    • 11
    • 7
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    DDIVAPITHV SVTLDGIPEV METQQVQETN ADSPGASSPE QRKRKKGRKT
    Predicted Reactivity
    Dog: 80%, Horse: 80%, Human: 87%, Mouse: 100%, Rabbit: 87%, Rat: 88%
    Characteristics
    This is a rabbit polyclonal antibody against ELF1. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of mouse ELF1
    Top Product
    Discover our top product ELF1 Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 612 AA

    Restrictions
    For Research Use only
  • Validation #104222 (Western Blotting)
    'Independent Validation' Badge
    by
    Dittmann Lab, Microbiology Department, NYU Langone Health, NYU School of Medicine
    No.
    #104222
    Date
    10/28/2019
    Antigen
    ELF1
    Lot Number
    66160414
    Method validated
    Western Blotting
    Positive Control

    A549 (endogenous ELF1), ELF1 recombinant protein

    Negative Control
    Notes

    Passed. ABIN2779831 specifically recognizes endogenous ELF1 in A549 cell lysates and recombinant human ELF1.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2779831
    Secondary Antibody
    goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234)
    Full Protocol
    • Grow A549 cells (ATCC, CCL-185) in DMEM medium (Corning, 10-013-CV) supplemented with 10% FBS (Atlanta biologicals, S11150) and Penicillin-Streptomycin (Corning, 30-002-CI), at 37°C and 5% CO2 to 3x104 cells/cm2 in 2ml on a 6 well plate (Costar, 3516).
    • Wash cells once with 1x PBS treat with trypsin (Corning, 25-053-CI).
    • Take cells up in growth medium.
    • Wash cells once with 1x PBS.
    • Resuspend approximately 2.05x105cells/well in 50-100µl 1x LDS sample buffer (Life Technologies, B0007). As positive control, take 20µg recombinant ELF1 (Abnova, H00001997-P01) in 1x LDS sample buffer.
    • Denature samples for 3min at 95°C and subsequently keep them on ice.
    • Separate samples on a Bolt 4-12% Bis-Tris Plus Gel (Invitrogen, NW04122) in an electrophoresis chamber (Mini Gel Tank, Invitrogen, A25977) for 30min at 80V and then for 60min at 120V.
    • Transfer proteins onto nitrocellulose membrane (Invitrogen, IB23002) using an iBlot 2 Gel Transfer Device (Invitrogen).
    • Block the membrane with TBS containing 0.05% Tween (TBST) containing 5% skim milk for 1h at RT.
    • Incubation with primary
      • o rabbit anti-ELF1 antibody (antibodies-online, ABIN2779831, 0066160414) diluted 1:500 in TBST containing 5% skim milk ON at 4°C.
      • loading control mouse anti-beta actin antibody (Invitrogen, MA5-15739) diluted 1:5000 in TBST containing 0.5% skim milk ON at 4°C.
    • Wash membrane 3x for 10min with TBST.
    • Incubation with secondary
      • goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
      • goat anti-mouse HRP-conjugated antibody (Invitrogen, G21040) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
    • Wash membrane 3x for 10min with TBST.
    • Reveal protein bands using SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific, 34075) and a ChemiDoc MP Imaging System (Bio-Rad).
    Experimental Notes
    • ABIN2779831 reveals a protein with an apparent molecular weight of the expected MW of 67kDa for ELF1 and some extraneous bands of lower intensity. ABIN2779831 also reveals a recombinant GST-tagged protein at the expected molecular weight (theoretical MW 93.9kDa).

    • Other ELF1-antibody dilutions were tested, but a dilution of 1:5000 was found optimal in terms of minimal background and strength of signal.

  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
    Alternative Name
    ELF1 (ELF1 Products)
    Synonyms
    zef1 antibody, fb05d07 antibody, wu:fb05d07 antibody, Elf-1 antibody, Sts1 antibody, mElf-1 antibody, p70 antibody, xelf1 antibody, elf-1 antibody, E74 like ETS transcription factor 1 antibody, E74-like ETS transcription factor 1 antibody, E74-like factor 1 antibody, E74 like ETS transcription factor 1 L homeolog antibody, E74 like ETS transcription factor 1 S homeolog antibody, ELF1 antibody, elf1 antibody, Elf1 antibody, elf1.L antibody, elf1.S antibody
    Background
    Elf1 belongs to the ETS family. Elf1 is a transcription factor that activates the LYN and BLK promoters. Elf1 may interact with other transcription factors in order to regulate specific genes. Elf1 can bind to the underphosphorylated form of RB.
    Alias Symbols: p70, Sts1, Elf-1, mElf-1
    Protein Interaction Partner: Ephb4, Dmd, Jak2, Foxp3,
    Protein Size: 612
    Molecular Weight
    67 kDa
    Gene ID
    13709
    NCBI Accession
    NM_007920, NP_031946
    UniProt
    Q60775
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