HIRA antibody (Middle Region)

Catalog No. ABIN2779934

Product Details anti-HIRA Antibody

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIRA antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LRSRELESSS DSDEKVHLAK PSSLSKRKLE LEVETVEKKK KGRPRKDSRL

Predicted Reactivity: Dog: 79%, Guinea Pig: 93%, Horse: 79%, Human: 86%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Hira. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of mouse Hira

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1015 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: Hira (HIRA Products)

Synonyms: HIRA antibody, wu:fb97b07 antibody, wu:fi43b08 antibody, si:ch211-188g24.3 antibody, AA138857 antibody, D16Ertd95e antibody, N28177 antibody, Tuple1 antibody, DGCR1 antibody, TUP1 antibody, TUPLE1 antibody, dgcr1 antibody, hira-A antibody, tup1 antibody, tuple1 antibody, homolog of histone chaperone HIRA antibody, histone cell cycle regulator antibody, histone cell cycle regulator a antibody, protein HIRA antibody, HIR histone cell cycle regulation defective homolog A (S. cerevisiae) antibody, HIRA antibody, histone cell cycle regulator S homeolog antibody, histone chaperone HIRA-like protein antibody, Tuple1/HirA antibody, HIRA antibody, hira antibody, LOC100052845 antibody, LOC100352472 antibody, LOC100407742 antibody, LOC100450459 antibody, LOC100467668 antibody, LOC100581135 antibody, LOC486424 antibody, Hira antibody, LOC732736 antibody, hira.S antibody

Background: Hira is required for the periodic repression of histone gene transcription during the cell cycle.Hira cooperates with ASF1A to promote replication-independent chromatin assembly.Hira is required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Alias Symbols: AA138857, D16Ertd95e, N28177, Tuple1
Protein Interaction Partner: Nanog, Creb3l4, Crem, Pou5f1, Hirip3, Pax7, Pax3, H3f3b,
Protein Size: 1015

Molecular Weight: 112 kDa

Gene ID: 15260

NCBI Accession: NM_010435, NP_034565

UniProt: Q61666

Pathways: Chromatin Binding