FBXW7 antibody (Middle Region)

Catalog No. ABIN2780093

Product Details anti-FBXW7 Antibody

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: GIDEPLHIKR RKIIKPGFIH SPWKSAYIRQ HRIDTNWRRG ELKSPKVLKG

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 93%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against Fbxw7. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of mouse Fbxw7

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 629 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FBXW7 antibody (ABIN2780093)

Huang, Ma, Li, Yu, Zhang, Wei, Jin, Xu, Gao, Huang: "NF-κB1 inhibits c-Myc protein degradation through suppression of FBW7 expression." in: Oncotarget, Vol. 5, Issue 2, pp. 493-505, (2014) (PubMed).

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: Fbxw7 (FBXW7 Products)

Synonyms: cdc4 antibody, AGO antibody, CDC4 antibody, FBW6 antibody, FBW7 antibody, FBX30 antibody, FBXO30 antibody, FBXW6 antibody, SEL-10 antibody, SEL10 antibody, hAgo antibody, hCdc4 antibody, 1110001A17Rik antibody, Cdc4 antibody, Fbw7 antibody, Fbwd6 antibody, Fbx30 antibody, Fbxo30 antibody, Fbxw6 antibody, F-box and WD repeat domain containing 7 antibody, F-box and WD-40 domain protein 7 antibody, Fbxw7 antibody, FBXW7 antibody, fbxw7 antibody

Background: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
Alias Symbols: 1110001A17Rik, AGO, Cdc4, Fbw7, Fbwd6, Fbx30, Fbxo30, Fbxw6, SEL-10
Protein Interaction Partner: Skp1a, CREB3L1, CREB3L2, Cul1, Prkcz, Prkce, Prkcd, Prkcb, Prkca, Pten, Mcl1, CEBPA, Pin1, MYB, Npm1, Jun,
Protein Size: 629

Molecular Weight: 70 kDa

Gene ID: 50754

NCBI Accession: NM_080428, NP_536353

UniProt: Q8VBV4

Pathways: Notch Signaling, EGFR Signaling Pathway