TOP2B antibody (Middle Region)

Catalog No. ABIN2780351

Product Details anti-TOP2B Antibody

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ENEGDYNPGR KTSKTTSKKP KKTSFDQDSD VDIFPSDFPT EPPSLPRTGR

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TOP2B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TOP2B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1621 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TOP2B

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Alternative Name: TOP2B (TOP2B Products)

Synonyms: TOP2B antibody, D230016L12Rik antibody, Top-2 antibody, TOPIIB antibody, top2beta antibody, DNA topoisomerase II beta antibody, topoisomerase (DNA) II beta antibody, microRNA 4442 antibody, TOP2B antibody, Top2b antibody, MIR4442 antibody

Background: TOP2B is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants, however, the second variant has not yet been fully described. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: TOPIIB, top2beta
Protein Interaction Partner: BAZ1B, SUMO2, SUMO3, UBC, EIF6, RPA3, RPA2, RPA1, ERG, WHSC1, NOTCH1, ESR1, NCSTN, SNRNP200, RBM14, HNRNPR, HDAC3, XPC, TOP2A, SNRPE, SMARCE1, SMARCD2, SMARCC2, SMARCC1, SMARCB1, SMARCA4, SMARCA2, SFPQ, S100A9, MSH2, IK, SEPT7, SUMO1, CUL5, NEDD8, RANBP2,
Protein Size: 1621

Molecular Weight: 183 kDa

Gene ID: 7155

NCBI Accession: NM_001068, NP_001059

UniProt: Q02880